top of page
  • Writer's pictureNanoEntek

About HEK293 Cells

Origin

One of the most extensively utilized cell lines is HEK293 cells, which are immortalized cells that originate from human embryonic kidney cells. HEK from HEK293 cells refers to Human Embryonic Kidney, and 293 represents the 293rd experiment performed by Frank Graham, who established the HEK293 cell line. This was not derived from a cancer cell but was immortalized through transfection with sheared adenovirus 5 DNA.

HEK293 cells

Characteristics

HEK293 has an average diameter of 11-15 μm. It is defined as a hypotriploid, indicating that it has fewer than three times the amount of chromosomes as a haploid.


HEK293 has a variant form, known as HEK293T. HEK293T is derived from the parent cell line HEK293 and hence T refers to the SV40 large T antigen (TAg), which optimizes transfection efficiency. As a result, hek293T is often used to replicate a large number of recombinant proteins or retroviruses.


Some advantages of HEK293 are as follows:

  1. Relatively fast and massive growth (doubling time of 36 hours) - hence efficiently used in massive transfection and recombinant proteins at high concentration.

  2. Low cost maintenance - can grow under serum-free environment and in suspension.

  3. Relatively low expression of ion channels and receptors - making it helpful for studying membrane receptors and ion channels.

  4. A "packaging cell" - HEK293 carries the retroviral vector containing target gene into the retroviral particles for gene replication


Culturing conditions

In 37℃ and 5% CO2 condition in an incubator


Media : high glucose growth media such as DMEM (Dulbecco’s Modified Eagle Medium) with antibiotic (penicillin) added to prevent contamination.


Usage

Aside from massive transfection and recombinant proteins, HEK293 cells are used in cancer research, protein interactions, drug testing, and other biological applications.



NanoEntek

 

Introducing NanoEntek latest automated cell counters


ADAM MC Plus
ADAM MC Plus

Cell types

Cell lines, primary cells

Measuring range

5x10E4 - 2x10E7 4x10E5 - 1x10E7 (optimal)

Loading volume

15 μL

Staining reagent

Acridine orange / DAPI

ADAM CellT Plus
ADAM CellT Plus

Cell types

Cell lines, primary cells

Measuring range

5x10E4 - 2x10E7 4x10E5 - 1x10E7 (optimal)

Loading volume

15 μL

Staining reagent

Acridine orange / DAPI

21 CFR Part 11

Compliant

NanoEntek also provides a live cell imaging system


JuLI Stage
JuLI Stage

Objective lens

4x, 10x, 20x

Channel type

Brightfield, GFP, RFP, DAPI

Vessel type

Well plate, flask, dish, slide

Incubator installation

Available





31 views

Recent Posts

See All

Comments


Commenting has been turned off.
bottom of page