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FREND is a device designed to quantitatively measure specific antibodies in human plasma and serum using the FREND cartridge. Commonly asked questions are listed below. Find the information that you are looking for. Check the list step by step. If you have more questions other than the lists, please let us know. 


Learn more about FREND by asking the following questions!

  • What is FREND System?
    The FREND is an automated, compact cartridge analysis instrument. It is based on quantitative immunoassay technology, enables quantitative measurement of single or multiple samples through a laser fluorescence cartridge. FREND cartridges utilize microfluidic techniques which controls the fluidity of the sample in the cartridge. In short, the concentration of the unknown sample is determined according to the ratio of the fluorescence intensity of the test and standard regions.
  • What are the technologies used in the FREND?
    FREND utilizes representative 4 technologies to enable the point-of-care-testing (POCT). Bio-MEMS Microfluidics Surface treatment Plastic Injection Molding
  • What are the principles behind the FREND?
    It is an automated, compact cartridge analysis equipment that enables quantitative measurement of single or multiple samples through a laser fluorescence cartridge based on quantitative immunoassay technology. Using an immunocomplex sample contained in the sample through microfluidic techniques, the concentration of the unknown sample is determined according to the ratio of fluorescence intensity of the test and standard regions.
  • What are the applicable items used in the FREND?
    You can find FREND items in about 5 categories: Urology, Thyroid (Internal Medicine), Cardiovascular, Respiratory, and General Health. Urology PSA, Testosterone Thyroid TSH, Free T4, Total T3, Thyroid Duo (TSH / Free T4) Cardiovascular PCT, BNP, Troponin I, Cardiac Triple (TnI, CK-MB, Myoglobin), D-dimer, hsCRP Respiratory FLU A&B, COVID-19 Ag, COVID-19 Ag + FLU A&B, COVID-19 Total Ab, COVID-19 IgG / IgM Duo, COVID-19 SP, COVID-19 Neutralization Ab General Health Vitamin D
  • What will FREND be able to do for users?
    1. Promptness: By improving the inspection efficiency in hospitals and clinics with quick and easy inspection procedures, patient satisfaction and hospital profitability can be expected to increase. Inspection results can be checked within 3 to 10 minutes, and the inspection can be completed in 3 simple steps. 2. Convenience: The intuitive touch screen GUI provides the convenience of using the equipment, and the stored patient information can be easily transferred to a PC using USB. 3. Updates: Periodic updates are being made to improve the accuracy of product analysis. FREND focuses on customer convenience by reflecting customer opinions.
  • Whys is FREND system so reliable?
    FREND system has been recognized for its cutting-edge technologies. More than 20 countries around the world uses FREND system to determine medical decisions for patients. For major 5 items (PSA, TSH, Free T4, Testosterone, and Vitamin D), FREND system in the united state of America is helping the medical professionals make right decision for the patients. In Europe, Asia, and the Middle East as well as in the United States, FREND is helping patients prevent or track disease through several items.
  • Does FREND require additional maintenance?
    No. The main maintenance, such as periodical quality check, can be easily done by users through the provided QC cartridge (if required). Other than the QC testing, no additional maintenance is needed. (The additional maintenance may be asked depends on the regional circumstances - for more information, please consult with NanoEntek FREND Academic support team or regional consultant)
  • What is the storage condition for cartridges?
    All the cartridges should be stored at 2 to 8 Celsius (36 to 46 Fahrenheit). Leave the cartridges in the ambient temperature about 20 to 30 minutes before use.
  • What are the sample type tubes for the each sample? What is the maximum storage temperature and time for the blood samples per item?
    Depending on the test item, the sample type is different. Please check the following descriptions.
  • Does the condtion of the sample affect the experimental results?
    Yes. The experimental results may vary depending on the condition of the blood. For example, hemolysis, excessive fat, coagulated specimens, and contaminated blood may cause different results.
  • Which procedure is required before injecting the sample into the cartridge?
    The plasma should be separated by rotating in a centrifuge at 3,000 rpm for at least 10 minutes after taking a blood sample from the patient. It can be used in the FREND using the corresponding plasma.
  • What is the difference between serum and plasma?
    Overall, serum and plasma refer to the liquid remaining after blood cells are separated. Serum refers to the liquid after the blood has been clotted, and in the case of plasma, it refers to the liquid that does not clot after adding an anticoagulant.
  • What are the difference in EDTA, Heparin, and Sodium Citrate?
    All of them are categorized as anticoagulant. NanoEntek recommend that you use the appropriate anticoagulant for each item and apply it to the product. The type of anticoagulant can be identified by the color of the tube top. (Tube colors may vary between manufacturers.)
  • What are the precautions?
    Caution is required as different results may be obtained in the following situations: • Contaminated cartridges • Injected cartridge is abandoned over 15 minutes • Sample does not develop, but leave in “sample well” • Sample is touched with bare hands or clothes after the sample is injected • Cartridge is left in the ambient temperature over 15 minutes as opened or exposed to the deformation factors (sunlight, heat, and moisture) • Cartridge (and the components) were stored at abnormal temperature in a long term or suspected exposed in during the shipment • Expired cartridge • Damaged cartridge's barcorde
  • What is the best environments to install the FREND?
    Avoid areas where contaminants (dust, blood samples, etc.) exist. Wipe out before testing. Avoid places with the high humidity and direct sun exposure.
  • What is code chip? Why is it necessary?
    Code chip is a device designed to generate and store a calibration curve whenever a new LOT is manufactured. Variation between Lots can be prevented through calibration, and the user does not need to perform separate calibration at all. Please, register the code chip in the FREND before using the device.
  • What is QC cartridge? When do I need to use it?
    QC cartridge is a device designed to perform quality checks such as laser power level, laser alignment and calculation ratio in the device. You can check the status of the product using the QC code chip and QC cartridge, and the result displays on the screen. It is recommended to use the system check through the QC cartridge every day
  • Patient status
    Please note the patient’s status or medication history in order to make a clear clinical decision before testing the test.
  • I have a new box. What should I do first?
    Check all the contents are enclosed. Take out the codechip and install it before doing a test.
  • How to use the pipette?
    Reverse pipetting is recommended when loading samples into cartridges when using the FREND. Compared to the conventional pipetting, it is possible to inject an accurate volume and prevents the generation of air bubbles. NanoEntek recommends to use the provided pipette which is enclosed with the FREND device.
  • How can I load the sample in the pipette?
    Make sure that the pipette contains the correct volume when transferring samples using the pipette.
  • Is there a way to check the cartridge sample flow?
    You can check the cartridge sample flow completion by looking at the backside of the cartridge.
  • Can I re-read the used cartridge?
    Yes. Results can be obtainedwithin 20 minutes of sample injection. However, we do not recommend it. Please use a new cartridge.
  • What is the sample loading volume?
    The proper volume for the FREND is 35μL. The error range is ±5μL, but it is not recommended to arbitrarily load or remove the loaded sample.
  • When and how to use the blocking reagent?
  • What is the reference range for each item?
  • How can I adjust the device factor setting?
    The device factor is for manipulating the systematic data calculation, if the conversion is necessary for the regional setting issue. It is not recommended to change the values without any consultation to NanoEntek technical support team.
  • The patient's numerical interpretation is different than expected. Why is that?
    The number may vary depending on the patient's condition and the type of medication taken. For more detailed information, please contact the academic team for further information.
  • There is a pop up message starts with EMTF. What is it?
    EMTF error occurs when the test conditions does not fulfill the criteria. For example, EMTF-01 error messages appear when the inserted cartridge number does not match with the installed code chip information. Please, check the manual FREND uploaded in NanoEntek homepage.
  • Canceling the AP machine
    Press the next button more than 7 seconds. It will automatically cancel the procedure.
  • How to fix the AP dropping error?
    There are three options that you can check before contacting to NanoEntek. (1) Pipette setting is not 35μL. The incorrect volume will cause the loading issue on pretreatment tube. (2) Please load the sample at the bottom of pretreatment tube. Less volume of sample will be loaded, if the sample remains on the tube wall. (3) This is when the device does not operate normally due to contaminants attached to the AP sensor. In this case, cleaning the AP sensor is necessary.
  • The error message says that the memory is full (EMDS-01). However, I can still perform the test? What is the problem?
    FREND can store upto 1,000 patient data in the storage. If you encounter the error message, it means that you need to delete all the data. FREND system is programmed that you can still conduct the test, even though the memory is full (But, the test result is not saved). Please, back up the data (if needed), and do the entire data deletion to secure the storage.
  • How can I dissemble the device?
    NanoEntek does not recommend temporary disassembly or modification of the product. If there is an error with the device or if you decide that repair is necessary for the malfunction, please contact the sales team and technical support team for repair instructions.
  • A gray screen appears and an error occurs after a software update.
    These phenomena can be classified into two categories. (1) If a gray screen appears during booting and there is no further program progress, it is necessary to check the device and install the program overall. In this case, please contact our sales team or technical support team for guidance regarding repair. (2) If booting has progressed, but a gray screen appears in a specific part, the file is corrupted due to forcible USB disconnection or an error during software update. Please reinstall the software.
  • We have our own LIS cable. Can we use it for the LIS connection?
    FREND system uses communication Interface RS232-C. The hard ware interface requires 4 pin mini DIN to serial Cable. The cable is customized to the system. Please contact to the sales team or technical support team to be guided for the LIS cable specification.
  • How can I use the internal QC material?
    Internal QC material is for in-house procedure to do the constant monitoring of the quality of the instruments and reagents by using Internal QC material (INQC). By doing so, the lab can monitor the accuracy and the precision of the complete analytical process.
  • The program does not initiate. The logo is running, but no more progress is seen.
    The error happens when your storage is full(or almost full) or there are too many data to process before initiating the program. If the program does not start automatically after waiting for enough time (ex. ≥ 30 minutes), please contact to NanoEntek support team. If the program initiates and asks to set the date and time again, please follow the instruction displays on the screen.

You can also check more information about FREND in our website (

[FREND System]

Rapid quantitative immunoassay analyzer


FREND system
3 kg
4 kg
240 x 260 x 175 mm
195 x 230 x 210 mm
3.33 A
5.0 A
DC 12 V
DC 12 V
Electrical Rating-Frequency
50 / 60 Hz
50 / 60 Hz
Electrical Rating-Current
1.7 A
1.7 A
Electrical Rating-Voltage
100 - 240 V
100 -240 V

Yes Easy-to-use | Yes Rapid (3 minutes) | Yes Small sample volume

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